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Image Search Results
Journal: Frontiers in Molecular Biosciences
Article Title: Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes
doi: 10.3389/fmolb.2022.843234
Figure Lengend Snippet: Expression validation of prognostic biomarkers in clinical samples. (A) Expressions of P4HA1, MLLT11, AURKA, and GOT1 in tumor and paratumor samples by RT-qPCR. (B) Expressions and quantification result of P4HA1, MLLT11, AURKA, and GOT1 in tumor and paratumor samples by Western blot. *, p < 0.05 vs. control; **, p < 0.01 vs. control; ***, p < 0.001; ****, p < 0.0001.
Article Snippet: Then, mRNA was reversely transcribed into cDNA using a PrimeScript RT reagent kit with gDNA Eraser (RR047A, Takara, Japan). qRT-PCR was performed using a GoTaq qPCR and
Techniques: Expressing, Quantitative RT-PCR, Western Blot